Literature summary extracted from

Zhao, Y.; Gutshall, L.; Jiang, H.; Baker, A.; Beil, E.; Obmolova, G.; Carton, J.; Taudte, S.; Amegadzie, B.
Two routes for production and purification of Fab fragments in biopharmaceutical discovery research: Papain digestion of mAb and transient expression in mammalian cells (2009), Protein Expr. Purif., 67, 182-189.

Application

EC Number	Application	Comment	Organism
3.4.22.2	synthesis	Fab antibody fragment production and purification by papain digestion of an intact monoclonal antibody. After digestion, separation of the Fab fragment from the Fc fragment and residual intact antibody is achieved using protein A affinity chromatography. The Fab fragments are of high quality suitable to produce diffraction quality crystals suitable for X-ray crystallographic analysis	Carica papaya

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.22.2	Carica papaya	-	-	-

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Release 2023.1 (January 2023)